Effects of Aqueous Dispersions of C60, C70 and Gd@C82 Fullerenes on Genes Involved in Oxidative Stress and Anti-Inflammatory Pathways

Background: Fullerenes and metallofullerenes can be considered promising nanopharmaceuticals themselves as a basis for chemical modification. As reactive oxygen species homeostasis plays vital role in cells, the study of their effect on genes involved oxidative stress anti-inflammatory responses are particular importance. Methods: Human fetal lung fibroblasts were incubated with aqueous dispersions C60, C70, Gd@C82 concentrations 5 nM 1.5 µM 1, 3, 24, 72 h. Cell viability, intracellular ROS, NOX4, NFκB, PRAR-γ, NRF2, heme oxygenase NAD(P)H quinone dehydrogenase 1 expression have been studied. Results & conclusion: The fullerenes active participants (ROS) homeostasis. Low high fullerene (AFD) similar effects. C70 was most inert substance, C60 substance. All AFDs both “prooxidant” “antioxidant” effects but different balance. substance more pronounced antioxidant properties, while had properties.